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    An allele sequence used in EcMLST is trimmed from the consensus sequence of the allele PCR product. It is obtained by aligning the consensus sequence with the reference sequence in K-12 (Table 1) and then trimming the sequence to the alignment length.

    Table 1: Reference sequences in K-12 for MLST of pathogenic E. coli
    Allele FASTA MEGA
    All 7 Alleles
    All 15 Alleles
    arcA
    aroE
    aspC
    clpX
    cyaA
    dnaG
    fadD
    grpE
    icdA
    lysP
    mdh
    mtlD
    mutS
    rpoS
    uidA

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    National Institutes of Health (NIH) National Institute of Allergy and Infectious Diseases (NIAID) Food and Waterborne Diseases Integrated Research Network (FWD IRN) Microbiology Research Unit (MRU) Reference Center to Facilitate the Study of Shiga Toxin-Producing Escherichia Coli