STEC Isolate Database Structure
STEC Center Isolate Database
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    Isolate
    Otype
    Htype
    Host
    Locale
    Date
    Clinical
    Source
    Strain name you are searching for (e.g. 86-24).
    Somatic antigen type (O serogroup, e.g. O26).
    Flagellar antigen type (H type, e.g. H11).
    Human, other animal (e.g. cow), or environmental (e.g. food) source.
    Country (state) in which the strains were originally isolated (e.g. USA)
    Year of the original isolation of the strain (e.g. 1975).
    Basic clinical information (e.g. 'D' = diarrhea; 'HUS' = hemolytic uremic syndrome).
    Investigator (e.g. Karch) or organization (e.g. CDC) who provided the strain.


    Alias Table
    Look up table of alternative labels for strains. Returns Whittam lab accession number (TW0000)


    Virulence Table
    Presence/absence of genes (pos/neg)
    These test are performed by gene probes or PCR
    Stx1
    Stx2
    Eae
    Ehec
    Ehly
    KatP
    EspP
    EtpD
    EAF
    Shiga toxin gene 1
    Shiga toxin gene 2
    intimin gene
    EHEC plasmid (pO157)
    Enterohemolysin gene
    catalase gene
    serine protease gene

    EAF (EPEC adherence factor) plasmid
    Alleles (allele labels)
    These data are based on sequencing or RFLP.
    Stxa1
    Stxa2
    Eae
    EspP
    allele designations (stx1a1,stx1a2, ..)
    allele designations (stx2a1,stx2a2, ..)
    allele designation (a-eae, b-eae, d-eae, g-eae)
    allele designations (A, B, C ..)
    Other positive and negative tests
    pf27
    iha
    TAI
    PCR detection of uidA+92 mutation (Feng)
    IrgA homolog adhesin gene (Tarr et al., in press)
    tellurite resistance and adherence island (Tarr et al., in press)
    Phenotypes
    VT
    Hemolysin
    Hep2
    FAS
    AE
    Sor
    Gud
    OtherPos
    OtherNeg
    Comments
    Cytotoxin expression detected in tissue culture
    Production of lysis zone on blood agar
    Pattern of adherence in cell culture (LA, local; DA, diffuse, AA autoaggregative)
    Tissue culture assay for actin (pos/neg), in 3h or 6h assay
    Attaching effacement observed in animal model (pos/neg)
    Ability to ferment sorbitol in less than 4h (pos/neg)
    Presence of absence of beta glucuronidase activity (pos/neg)





    ET Table
    Electromorph profiles from strains based on multilocus enzyme electrophoresis.

    Contact stec@cvm.msu.edu
    Operated by Shannon D. Manning at Michigan State University